Lupine Publishers | Scholarly Journal of Food and Nutrition
Abstract
Cholecalciferol is a steroid hormone
(7-dehydroxycholesterol), which helps in the absorption of dietary minerals
like zinc, calcium, magnesium, iron, and phosphate and also responsible for
other biological activity. In this research study, the influence of the Trivedi
Effect®-Consciousness Energy Healing Treatment on the physicochemical and
thermal properties of cholecalciferol was evaluated using the modern analytical
technique. The cholecalciferol test sample was divided into two parts. One of
the test samples was considered as a control sample, which did not receive the
Biofield Energy Treatment; whereas, the other part was treated with the
Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy
Healer, Gopal Nayak and termed as a treated sample. The particle size values in
the treated cholecalciferol powder sample were significantly decreased by 78.94%
(d10), 26.21% (d50), 22.01% (d90), and 29.04%
{D (4,3)}; thus, the specific surface area of the treated cholecalciferol was
significantly increased by 174.12% compared to the control sample. The XRD peak
intensities and crystallite sizes of the treated cholecalciferol powder sample
were significantly altered ranging from -44.37% to 370.49% and -74.48% to
91.52%, respectively; therefore, the average crystallite size of the treated
cholecalciferol was significantly decreased by 36.21% compared to the control
cholecalciferol. The latent heat of fusion of the treated cholecalciferol was
significantly increased by 25.89% compared to the control cholecalciferol. The
weight loss was decreased by 1.48%; whereas, the residue amount was
significantly increased by 107.02% in the treated sample compared with the
control sample. Thus, the Trivedi Effect®-Consciousness Energy Healing
Treatment generated a new polymorphic form of cholecalciferol which might offer
better solubility, absorption, bioavailability, and be thermally more stable
compared with the control sample. Henceforth, the Consciousness Energy Healing
Treated cholecalciferol would be more beneficial to maintain the overall
quality of life and it would be more useful in designing novel
nutraceutical/pharmaceutical formulations for the better therapeutic responses
against deficiency of vitamin D, osteoporosis, rickets, cardiovascular
diseases, diabetes mellitus, cancer, mental disorders, multiple sclerosis, etc.
Keywords: Complementary and Alternative Medicine; Vitamin D3;
The Trivedi Effect®; Consciousness Energy Healing Treatment; Particle size;
Surface area; PXRD; DSC; TGA/DTG
Introduction
Vitamin D3
(cholecalciferol or 7-dehydroxycholesterol) is a steroid hormone produced in
the skin once exposed to ultraviolet light and also obtained from dietary
sources. It is a fat-soluble vitamin helps in the absorption of the dietary
minerals like zinc, calcium, magnesium, iron, and phosphate and responsible for
other biological activity, i.e., maintain healthy immune, skeletal,
cardiovascular, and reproductive systems [1-3]. The major dietary sources of
vitamin D are the cod liver oil, fatty fish like salmon and tuna, milk,
nutraceutical and pharmaceutical supplements [2]. It is used for the prevention
and treatment of several diseases like hypovitaminosis D, rickets,
osteoporosis, diabetes mellitus, cardiovascular diseases, mental disorders,
infections, cancer, multiple sclerosis, etc. [4-6]. Cholecalciferol nutritional
deficiency mostly under-diagnosed and under-treated is pandemic all over the
world [7]. The 1,25-Dihydroxycholecalciferol is the biologically active form of
cholecalciferol known as calcitriol [5- 7]. The overdosing of cholecalciferol
can be the cause of vomiting, constipation, hypercalcemia, polyuria, polydipsia,
insomnia, confusion, kidney stone, weakness, and mental retardation [1].
The bioavailability of
cholecalciferol is very poor. Similarly, some of the other factors that
directly affect the bioavailability of cholecalciferol are dietary fiber,
genetic factors, and cholecalciferol status [8,9]. The stability, solubility,
and bioavailability of cholecalciferol are the major concern for the storage
and nutraceutical/pharmaceutical formulations point of views, as it is
insoluble to water and sensitive to the light and air [10,11]. The
pharmaceutical and nutraceutical scientists are working hard for the
improvement of physicochemical properties of the compounds for better
dissolution, absorption, and bioavailability in the body [12]. In this concern,
the Consciousness Energy Healing Treatment (the Trivedi Effect®) has been
proved experimentally that, it has a substantial impact on various
physicochemical properties and also bioavailability of nutraceutical and
pharmaceutical entities [13-16]. The Trivedi Effect® is natural and only proven
scientific phenomenon in which a specialist can harness this intelligent energy
from the “Universe” and transfer it anywhere on the planet through the possible
mediation of neutrinos [17]. Around the body of every living organism an
infinite and para-dimensional unique electromagnetic field exists created from
the continuous moment of the charged particles like ions, cells, blood, etc. is
called a “Biofield”. The Biofield Energy Therapies have been reported with
significantly beneficial outcomes against various disease [18]. The National
Center for Complementary and Alternative Medicine (NCCAM) and the National
Institutes of Health (NIH) have recommend and included the Energy therapy under
the Complementary and Alternative Medicine (CAM) along with other therapies,
i.e., homeopathy, acupuncture, Ayurvedic medicine, naturopathy, acupressure,
Tai Chi, Qi Gong, Reiki, hypnotherapy, etc. The CAM has been accepted by most
of the USA population [19,20]. On the other hand, the Consciousness Energy
Healing Treatment (the Trivedi Effect®) has gained popularity all over the
world and reported with the substantial impact on the physicochemical and
behavioural properties of metals, ceramics, polymer, organic compounds,
microorganisms, cancer cells, crops, etc. [21-31]. In this study the impact of
the Trivedi Effect®-Consciousness Energy Healing Treatment on the
physicochemical and thermal properties of cholecalciferol was evaluated using
particle size analysis (PSA), powder X-ray diffraction (PXRD), differential
scanning calorimetry (DSC), and differential thermogravimetric analysis (DTG)/
thermogravimetric analysis (TGA).
Materials and Methods
Chemicals
and Reagents
The cholecalciferol powder sample
(> 98%) was purchased from Sigma-Aldrich, India and reaming chemicals were
of analytical grade purchased in India. Consciousness Energy Healing Treatment
Strategies The test sample vitamin D3 was equally divided into two
parts. One part of the vitamin D3 sample was received the
Consciousness Energy Healing Treatment (the Trivedi Effect®) remotely provided
standard laboratory conditions for 3 minutes by a well-known Biofield Energy
Healer, Gopal Nayak, India, known as a Biofield Energy Treated sample. Though,
the second part of vitamin D3 sample did not receive the
Consciousness Energy Healing Treatment but treated with a “sham” healer called
as a control sample. The “sham” healer doesn’t know anything about the Biofield
Energy Treatment. After the treatment, both the samples were kept in sealed conditions
and characterized using modern analytical techniques.
Characterization
The PSA of vitamin D3 was performed using Malvern Mastersizer 2000 (the UK) using the wet method [32,33]. The PXRD analysis of vitamin D3 powder sample was performed with the help of Rigaku Mini Flex-II Desktop X-ray diffractometer (Japan) [34,35]. The crystallites size was calculated from XRD data using the Scherrer’s formula (1)
Where k is the equipment constant, G
is the crystallite size in nm, λ is the radiation wavelength, β is the
full-width at half maximum, and θ is the Bragg angle [36,37].
Similarly, the DSC analysis of vitamin D3 was performed with the help of DSC Q200, TA instruments. The TGA/DTG thermograms of vitamin D3 were obtained with the help of TGA Q50 TA instruments [32,33]. The % change in particle size, specific surface area, peak intensity, crystallite size, latent heat, melting point, weight loss and the maximum thermal temperature of the Biofield Energy Treated vitamin D3 was calculated compared with the control vitamin D3 using the following equation 2:
Results and Discussion
Particle
Size Analysis (PSA)
The particle sizes of the control
and Biofield Energy Treated cholecalciferol were analyzed, and the data are
presented in Table 1. The particle size values in the treated cholecalciferol
were significantly decreased by 78.94%, 26.21%, 22.01%, and 29.04% at d10,
d50, d90, and D (4,3), respectively compared to the
control cholecalciferol. Therefore, the specific surface area of the Biofield
Energy Treated cholecalciferol (0.0625 m2/g) was significantly increased by
174.12% compared to the control sample (0.0228 m2/g). The results indicated
that the Trivedi Effect®-Consciousness Energy Healing Treatment supposed to act
as an external force for breaking the larger particles of cholecalciferol to
smaller one; hence increased the surface area. The particle properties (size,
shape, and surface area) of a drug molecule have a significant impact on the
solubility, absorption, bioavailability, and the therapeutic efficacy [12, 38].
As cholecalciferol is a lipophilic compound and the solubility of it is very
poor in water accountable for the poor bioavailability in the body [8,9]. The
Consciousness Energy Healing Treated cholecalciferol may show better
solubility, absorption, and therapeutic efficacy in the body.
Powder
X-Ray Diffraction (PXRD) Analysis
The PXRD diffractograms of both the
samples showed sharp and intense peaks (Figure 1), indicated that both the
samples were crystalline. The control and Biofield Energy Treated
cholecalciferol samples showed the highest peak intensity at 2θ equal to 17.76°
and 17.97° in the powder XRD diffractograms (Table 2). The peak intensities of
the treated cholecalciferol were significantly altered ranging from -44.37% to
370.49% compared to the control sample. Similarly, the crystallite sizes of the
treated cholecalciferol sample were significantly altered ranging from -74.48%
to 91.52% compared to the control sample. Largely, the average crystallite size
of the Biofield Energy Treated cholecalciferol (246.75 nm) was significantly
decreased by 36.21% compared with the control sample (386.8 nm). The change in
the peak intensity of the crystalline compound indicated the alterations in the
crystal morphology [38]. The alterations in the powder XRD pattern provide the
proof of polymorphic transitions [39,40]. The Trivedi Effect®-Consciousness
Energy Healing Treatment assumed to be responsible for the new polymorphic form
of cholecalciferol probably through the Consciousness Energy via neutrino
oscillations [17]. The drug performance, i.e., bioavailability, therapeutic
efficacy, and toxicity of the pharmaceutical compound would be different from
the original one due to different polymorphic forms [41-43]. Hence, the
Consciousness Energy Healing Treated sample would be more efficacious novel
pharmaceutical/nutraceutical formulations for the treatment of cholecalciferol
deficiency disease.
Differential
Scanning Calorimetry (DSC) Analysis
The cholecalciferol control and
treated samples showed the sharp endothermic peak in the thermograms (Figure
2). The literature data closely match the experimental results [42]. The
melting point and latent heat of fusion (ΔH fusion) of the Biofield Energy
Treated cholecalciferol were increased by 0.38% and 25.89%, respectively
compared with the control sample (Table 3). The change in the latent heat of
fusion can be directly related to the disrupted intrinsic molecular chain and
the crystal structure [43]. As per the result the thermal stability of the
Consciousness Energy Healing Treated cholecalciferol was improved compared to
the thermal stability of the control sample.
Thermal
Gravimetric Analysis (TGA) / Differential Thermogravimetric Analysis (DTG)
The control and Biofield Energy
Treated samples showed one step of thermal degradation (Figure 3). The weight
loss of the Biofield Energy Treated cholecalciferol was decreased by 1.48%;
whereas, the residue amount was significantly increased by 107.02% compared to
the control sample (Table 4). Similarly, the control and Biofield Energy
Treated cholecalciferol showed one peak of maximum thermal degradation
temperature (Tmax) in the thermograms (Figure 4). The Tmax
of the treated sample (292.01°C) was increased by 0.74% compared to the control
sample (289.87°C). Overall, thermal analysis data of cholecalciferol samples
indicated that the thermal stability of the Biofield Energy Treated sample was
increased compared with the control sample.
Conclusions
The Consciousness Energy Healing
Treatment (the Trivedi Effect®) has shown the significant effects on the
particle and crystallite size, surface area, peak intensity, and thermal
behavior of cholecalciferol. The particle size values in the Biofield Energy
Treated cholecalciferol powder sample were significantly decreased by 78.94% (d10),
26.21% (d50), 22.01% (d90), and 29.04% {D (4,3)}; thus,
the specific surface area of the Biofield Energy Treated cholecalciferol was
significantly increased by 174.12% compared to the control cholecalciferol. The
powder XRD peak intensities and crystallite sizes of the Biofield Energy
Treated cholecalciferol powder sample were significantly altered ranging from
-44.37% to 370.49% and -74.48% to 91.52%, respectively; therefore, the average
crystallite size of the Biofield Energy Treated sample was significantly
decreased by 36.21% compared with the control sample. The ΔH fusion of the
Biofield Energy Treated cholecalciferol was significantly increased by 25.89%
compared to the control cholecalciferol. The total weight loss was decreased by
1.48%; whereas, the residue amount was significantly increased by 107.02% in
the Biofield Energy Treated sample compared with the control sample. Thus, the
Trivedi Effect®-Consciousness Energy Healing Treatment generated a new
polymorphic form of cholecalciferol which might offer better solubility,
absorption, bioavailability, and be thermally more stable compared with the
control sample. Henceforth, the Consciousness Energy Healing Treated
cholecalciferol would be more beneficial to maintain the overall quality of
life and it would be more useful in designing novel
nutraceutical/pharmaceutical formulations for the better therapeutic responses
against deficiency of vitamin D, osteoporosis, rickets, cardiovascular
diseases, diabetes mellitus, cancer, mental disorders, multiple sclerosis, etc.
Acknowledgements
The authors are grateful to Central
Leather Research Institute, SIPRA Lab. Ltd., Trivedi Science, Trivedi Global,
Inc., Trivedi Testimonials, and Trivedi Master Wellness for their assistance
and support during this work
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