Showing posts with label Journal of Food and Nutrition. Show all posts
Showing posts with label Journal of Food and Nutrition. Show all posts

Friday, July 9, 2021

Lupine Publishers | Influence of Psychosocial Factors on Optimal Dietary Intake of University Students of Kenya

 Lupine Publishers | Scholarly journal of Food and Nutrition

Abstract

Background: University students’ encounter environmental changes which exerts mixed perceptions on sufficiency of their diets.

Objective: This cross-sectional study determined the influence of psychosocial factors influencing optimal dietary intake of university students.

Methods: This was conducted from August to November 2017 within 5 Kenyan universities purposively sampled. Stratified sampling was utilized to obtain 230 respondents. Quantitative data was collected where exploratory factor analysis tested dimensionality of questions, while skewness and kurtosis assessed normality of data. Structural equation modelling determined predictive power of latent variables.

Results: The model fitted data acceptably well; P<0.001, Tucker Lewis index =0.93, comparative fit index =0.95, root mean square error of approximation =0.090, Hoelter critical N (0.01=230], with optimal dietary intake. Regression weights showed predictive power for student’s attitude (β=0.68, P<0.01), subjective norm (β=0.36, P<0.05), perceived behavioural control (β=0.34, P<0.05) towards intention (β=0.95 P<0.001).

Conclusion: Student’s attitude was a significant factor in improving and upholding optimal dietary intake.

Keywords: Optimal dietary intake; Psychosocial factors; University students; Theory of planned behaviour

Introduction

Sub-optimal dietary habits are among the major risk factors for obesity, undernourishment, micro-nutrient deficiencies and associated metabolic conditions, particularly if adopted during early adulthood [1,2]. This is becoming more prevalent across all age groups where traditional healthy diets are being progressively replaced by more westernized dietary patterns [3]. University students seem to be the most affected by this nutrition transition [4]; studies have shown that young adults living away from home to attend university, experience numerous health-related behavioural changes, including the adoption of unhealthy dietary habits [5- 7]. These behaviours are mostly attributed to drastic changes in the environment and resources available, frequent exposure to unhealthy foods and habits. This leads to higher consumption of energy-dense foods and low intake of nutrient-dense foods in addition to frequent skipping of meals. Nutritionists and dieticians’ support have been reported to be an essential motivational factor in promotion of optimal dietary habit across populations of all age groups; however, evidence suggests this is not basically the case for university students.

For university students to be inspired to practice healthy dietary habits, the optimal balance between factors that manipulate their cognitive and experiential aspects must be achieved. These cognitive and experiential aspects are the psychosocial factors that determine student’s attitude, subjective norm and perceived behavioural control towards intention to practice healthy behaviour in this case optimal dietary intake. Intention is a significant predictor of dietary intake, which can be optimal or suboptimal. Studies have described many factors associated with the intention to practice optimal dietary intake amongst university students. These factors include environmental, socio- demographic and behavioural that determines students’ decision to optimal dietary intake [8-10]. However, there is limited information on psychosocial factors which include attitude, subjective norm and perceived behavioural control in association to practice of optimal dietary intake [11]. Thus, this current study aimed at revealing the influence of student’s attitude, subjective norm, perceived behavioural control on their intention to practice optimal dietary intake.

Methods and Data

Study Framework

The study adapted Ajzen’s Theory of Planned Behaviour (TPB) model [12] (Figure 1) to fit psychosocial factors that influence optimal dietary intake of university students. The TPB model works on the basis that the approach to target behaviour is to assess behavioural intention, which in turn is seen to be an operation of 4 exogenous variables: attitudes, subjective norm, perceived control and intention. In this context, students’ attitude was the certainty about likely outcomes of optimal dietary intake multiplied by evaluation of these outcomes (behavioural beliefs). Subjective norm was the belief of the students over important people in his/her life that may or may not have influenced them to optimally feed multiplied by the level of compliance to such influences (normative beliefs). Perceived behavioural control was viewed as control factors to promote or inhibit the students to optimally feed multiplied by the power they had over those factors (control beliefs). Perceived behavioural control was measured both directly and indirectly since optimal dietary intake was not under completely volitional control. Therefore, students’ attitude and subjective norm were posited to have influenced optimal dietary intake indirectly through intention.

While perceived behavioural control both indirectly and directly manipulated optimal dietary intake. Intention was described as a behavioural tendency that captured the motivational factors that had an impact on behaviour (optimal dietary intake). The latent variables were endogenous since they depend on observed variables to be measured, whereas observed variables were exogenous since they are independent variables. When a variable is believed to “cause” another variable, the relationship between the variables is shown as a directed arrow, from cause to effect. Whether one variable “causes” another is an assumption that the researcher makes and only data can reveal. Co variation between 2 variables is shown as a 2-headed arrow connecting the variables. As a theory of competency and mastery, psychosocial factors influencing students’ optimal dietary intake describes that initiation and persistence towards it are determined primarily by students’ optimal dietary intake cognitive judgments and expectations.

To comprehend optimal dietary intake using the TPB model [12], an elicitation study was initially conducted prior to the current study to elicit salient beliefs on which exogenous variables are based. This was then employed to construct a questionnaire, which was pretested and used to assess the influence of these psychosocial factors on optimal dietary of university students. Therefore, the Theory of Planned Behaviour model was the theory of focus since it has the ability to distinguish between those who perform and do not perform the behaviour under investigation [13]. Therefore, by utilizing the TPB, one can gain an understanding of the behaviour [optimal dietary intake] by tracing its determinants back to the underlying beliefs and possibly further influence the behaviour by changing a sufficient number of these beliefs [14]. A structural model was specified as per the objectives and tested as a good fit for data obtained on optimal dietary intake.

Study Area and Design

The study was conducted within 5 Public Kenyan Universities purposively selected with the aim that they had operational human nutrition programme that emphasized the health benefits of optimal dietary intakes. This cross-sectional study was conducted from August to November 2017 amongst 230 randomly selected and stratified students receiving education in a human nutrition related bachelor’s degree from the 5 universities. Permission was obtained from the School of Graduate Studies. Ethical approval was given by National Council for Science and Technology. Research authorization was granted by the respective administration of the 5 universities. We sought informed consent from the respondents who were informed on the research procedures, details and assured of confidentiality.

Sample Criteria and Sampling Techniques

Sampling procedures involved selection of universities and respondents. Purposive sampling was used to identify 5 Public Universities for the study with the target that they offered Human Nutrition related bachelor’s degree programme and were accredited by their regulator- Kenya Nutritionists and Dietetics Institute. Proportionate stratified sampling was used to get the sample size of respondents from each stratum of University A (46), University B (44), University C (50), University D (48) and University E (42). Random sampling was then employed on each stratum depending on its size to get the final study sample size of N=230. Sample criteria included both males and females’ students pursuing a bachelor’s degree in Human Nutrition. Participation in the study was voluntary although 4 % of respondents dropped out of the study due to inevitable circumstances but were immediately replaced through a random sampling procedure performed on eligible participants. The respondents were not compensated for taking part in the study but were highly appreciated and given a debriefing letter after completion of the interview sessions.

Data Collection Instrument

Data collection was conducted from August to November 2017 by the main researcher and 5 trained research assistants. A 7-point Likert-type scale optimal dietary intake questionnaire was used to collect the quantitative data. Both convergent and divergent validity were determined by comparing answers to each question measuring the same concept, then by measuring this answer to the respondent’s response to a question that asks for the exact opposite answer. Back-translation was also done to check for reliability of the translation. The pretested questionnaire was acceptable based on factor analysis criteria used and concepts of measurements loading with communalities for each item being greater than 0.5. This questionnaire was acceptable having confirmed adequacy of sample size (N=230) using test of sphericity. The internal consistency estimates of the various concepts, using Cronbach’s alpha, ranged from 0.64 to 0.89. The closer the Cronbach’s alpha coefficient is to 1.0 the greater the internal consistency of the items in the scale. George and Mallery’s rule of thumb was used to classify the Cronbach’s alpha coefficients generated.

Statistical Methods

Data was entered into SPSS version 15 to calculate for reliability tests where Cronbach’s alpha was used to assess the consistency of the questions. The response for each question was distinctive, and rotated component factors loading for each variable was subjected to principal component analysis. George and Mallery’s [15] rule of thumb was employed to categorize the average of communalities of student’s attitude, subjective norm, perceived behavioural control and intention. To establish whether the model nested based on TPB variables applied to optimal dietary intake fitted the data acceptably well, structural equation modelling using AMOS version 7 was conducted. Structural equation modelling was used to determine the influence of student’s attitude, subjective norm, perceived behavioural control and intention on optimal dietary intake. Intention was assessed by the extent to which respondents were willing to practice optimal dietary intake [eating a balanced diet, diet diversity & variety, and sufficient diets]. Measurements of this was done using a Likert-type scale ranging from 1 to 7, where 1 represented not at all and 7 represented very much. The overall model fit was evaluated using χ2 (CMIN) and relative χ2 divided by degrees of freedom (CMIN/df), comparative fit index (CFI), the standardized root means square error of approximation (RMSEA), Hoelter’s critical N, and Bollestine bootstrap. CFI and Tucker– Lewis index (TLI) values greater than 0.90 were considered satisfactory. RMSEA values less than 0.08 were considered satisfactory. CMIN/ df was regarded as fit when it ranged 3:1 and considered better when closer but not less than 1.0 Hoelter’s critical N for significance levels of 0.05 and 0.01 were used where bootstrap samples were set at 200.

Results

Influence of Student Attitude, Subjective Norm, and Perceived Behavioural Control on Intention to Practice Optimal Dietary Intake

It was found that the items characterizing attitude, subjective norm and perceived behavioural control had high regression weights approaching to 1.00. The relationships between observed variables in the model (Figure 2) were significant. The goodness of fit was statistically non-significant at the .01 level, but the model would be rejected at the 0.05 level (χ2 = 620.1, df = 250, P = 0.12, χ2 / df = 2.30). Although the χ2 was under the recommended 3:1 range indicating acceptable fit, after significant modification indices were unassociated. Other fit indices (TLI = 0.93, CFI = 0.95, RMSEA =0.090) also showed a good model fit (Table 1).

Note: RMSEA=Root mean square residual; CFI=Comparative fit index; CMIN/DF=Chi-square/degree of freedom; TLI= TuckerLewis Index; χ²= Chi-square.

Hoelter’s critical N values recommend that the model would have been accepted for lower limit at the .05 significance level with 200 cases, and the upper limit of N for the .01 significance level is 230 cases. The Bollen–Stine P =0.12 provided further reassurance about the model fit among other global fit indices. The regression weights (Figure 2) indicates that students’ attitude had a statistically significant influence on optimal dietary intake through intention (β= 0.68, P< 0.01, N=230). Indirect perceived behavioural control had a statistically significant influence on optimal dietary intake through intention (β=0.64, P<0.01, N=230). This was followed by subjective norm, which had a statistically significant influence on optimal dietary intake through intention (β= 0.52, P< 0.05, N=230). Intention was found to have the strongest prediction for optimal dietary intake (β = 0.95 P< .001, N = 230). Direct perceived behavioural control had the least influence on optimal dietary intake (β = 0.12, P> 0.05, N =230).

Discussion

This study focused on examining motivational beliefs [attitude, subjective norm and perceived behavioural control] associated with students practice of optimal dietary intake of based on the Theory of Planned Behaviour.

Influence of Student’s Attitude on Optimal Dietary Intake The default model had significant influence and was valued, since the forecasts supported the validity of the TPB model. The concept of influence involved dietary intake that was retrospectively explained by TPB, which allowed a prospective test of theoretical understanding. The influence of students’ attitude, subjective norm, perceived behavioural control and intention in extrapolating optimal dietary intake was tested. Standardized regression weights in (Figure 2) indicated that more students placed a high value on the health benefits of practicing optimal dietary intake (β = 0.68, P< 0.01, N = 230). Positive attitudes are associated with supportive significant others who motivated practice of optimal dietary intake [16]. Previously, revealed that associated health benefits were linked to practicing optimal dietary intake which had an impact on the attitudes of young adults in colleges. A recent study found that young adults, compared to middle-aged adults, had lower perception regarding optimal diets and health, suggesting a relatively low level of interest in health among young adults. Similarly, previous studies found that reasons for sub optimal dietary intake amongst the young adults were ‘poor attitudes’ and ‘wrong influence’. Thus, nutrition education, accurate information and right influence should positively impact on attitudes of students to practice optimal dietary intake based on one’s health concerns. In this study, students’ attitude was perceived to be associated with optimal dietary intake knowledge, influence of significant others and self-efficacy of the student. This illustrates that attitude correlates with other factors for it to be optimal. The current study established a statistically significant correlation between students’ attitude and subjective norm (r= 0.95, P= 0.001). The correlation between attitude and perceived behavioural control was stronger, positive and statistically significant (r = 0.97, P= 0.001). Contrary students may have positive attitudes towards optimal dietary intake although the obstacles they encounter surpass their ability to optimally practice the behaviour.

Influence of Student’s Subjective Norm on Optimal Dietary Intake

Subjective are the behavioural standards that exist in a social group for what is considered correct and appropriate behaviour [17], and they emerge from the shared practices and expectations of the group members. Previous research has shown that, through changing individual’ perceptions of existing social norms, various kinds of health behaviours can be influenced, such as optimal dietary intake [18]. A student’s decision to optimally feed is influenced by what is socially acceptable, open to social and cultural influences. We examined normative influences in relation to referents differing in social distance, including family members, friends, colleagues and mass media. However, of particular interest in this study was the detailed analysis of student’s perception of social pressure from significant sources of reference and change in perception of these views in relation to balanced diets, diet diversity & variety and diet sufficiency.

This finding suggests that informal groups such as family members, friends, colleagues and mass media are important sources to influence the practice of optimal dietary intake in students. Previous studies using the TPB have suggested somewhat inconsistent results regarding the influence of significant others, partly supporting the results of the current study [19,20]. Previously, [21,22] argued that a potential way to improve individual’s dietary intake was to intervene in the social norms that govern eating behaviour. Improving the social norms surrounding optimal dietary intake may stimulate healthy instead of unhealthy dietary intake of an individual. Fostering strong combined relationships amongst significant other is fundamental to support any individual practicing optimal dietary intake. When social and environmental support systems are in place, making healthful choices becomes possible and has an opportunity to improve dietary habits [23].

Influence of Student’s Perceived Behavioural Control on Optimal Dietary Intake

A statistically significant influence of indirect perceived behavioural control [β = 0.64, P <0.01, N = 230] and a statistically least significant direct perceived behavioural control [β = 0.12, P &li;0.05, N = 230] were reported. Ajzen’s contended that the direct link may only be apparent when perceived control closely parallels actual control. We conjecture that perceived control did not parallel actual control in this group of respondents, for whom the sense of dietary intake control only exerted its effect more indirectly through intentions. The indirect measure was promising because it showed that though students have other obstacles to optimal dietary intake, they have confidence in their ability to practice it. Previous research reported positive relationship between indirect perceived behavioural control and intention [24]. It was hypothesized that perceived dietary intake control would influence optimal dietary intake as it would be similar to self- efficacy, since both are concerned with perceived ability to perform a behaviour. When a student has higher sense of self-efficacy regarding optimal dietary intake, they will react more positively when problems arise and persist when confronted with problems. Therefore, a higher perceived behavioural control score in the indirect measure should be associated with lower problem severity perceptions in practice of optimal dietary intake.

Increasingly, younger Kenyans inclusive of university students have access to more education opportunities, consequentially higher possibilities for nutrition knowledge and their dietary intake habits. Based on the study analysis, student’s attitude, subjective norm, and indirect perceived behavioural control had a statistically significant influence on their intention of practicing optimal dietary intake. However, the direct influence of perceived behavioural control on optimal dietary intake illustrated a very minimal prediction (β = 0.12, P > 0.05, N = 230). As the analytical results of [24] showed that although direct perceived behavioural control is reasonably explained by belief control, it does not in turn provide better prediction of intention over and above that provided by indirect subjective norm, attitude, and perceived behavioural control. Furthermore, [25] also claimed that the direct perceived behavioural control is less likely to be related to intention. Given that the influence of student’s attitude, subjective norm and perceived behavioural control statistically influenced intention (β = 0.95, P < 0.001, N =230), this study established these psychosocial factors practically influences optimal dietary intake of students. This indicates that a unit change in student’s attitude, subjective norm and perceived behavioural control was associated with a change of 0.68, 0.52, 0.64 units respectively, in intention. A variance of 74% was obtained for intention predictors, whereas direct perceived behavioural control and intention accounted for 68% of variance on optimal dietary intake. Thus, the aspect of predictability of psychosocial factors is not just a mere postulation but a logically proven detail.

Implications for Practice and Research

Our data reveal that psychosocial factors remain statistically significant in influencing optimal dietary intake of university students. Since students desire to practice optimal dietary intake but they encounter barriers that originate from their attitude, social pressures from significant referents and their self-efficacy [confidence]. These attitudinal, normative, and control beliefs, which the study has determined to be predictive, are optimal dietary intake barriers/facilitators that limit students’ capability of practicing optimal dietary intake. Hence, efforts should include further promotion and research into students’ perception of the value of optimal dietary intake that affects their attitude. Significant others should address the issue of social support and influence optimal dietary intake intention. Discussing the students’ worries, providing education on the anxiety response and offering help with structured problem solving and sustainable techniques may help alleviate the students’ self-efficacy towards optimal dietary intake, thus boosting their emotional support. To enhance students’ selfefficacy, they need to be informed of the challenges that may arise during practice of optimal dietary intake and how to overcome them. Therefore, this research confirms that psychosocial factors, namely, students’ attitude, subjective norm, perceived behavioural control are important predictors of optimal dietary intake and may also hinder the practice of optimal dietary intake among young adults inclusive of university students.

Conclusion

Optimal dietary intake promoters, practitioners and researchers should capitalize on understanding and motivating young people including university students about these psychosocial factors that determine intention of optimal dietary intake by using the generated modified framework from Ajzen’s TPB. Since our findings support the use of the TPB to predict the influence of students’ attitude, subjective norm/social pressures and perceived behavioural control on optimal dietary intake intention, it is significant for programs to incorporate strategies that target individuals’ beliefs which in turn reveal what beliefs and factors need to be addressed for optimal dietary intake to be practiced. Although dietary intake of respondents in the 5 universities varied, they were only reported and controlled during analysis but not established. The analysis of dietary intake in each of the 5 universities could have been conducted by comparing model fitness indices; however, sample size did not allow for smaller clustering of respondents. This study recommends that educating university students about the benefits of optimal dietary intake, identifying barriers to optimal dietary intake, developing strategies to address the barriers and identifying the significant referents may be important areas to target for

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Friday, June 4, 2021

Lupine Publishers | Development and Application of HPLC/Post-Column Method for Analysis of Low Molecular Weight Organic Acids in Beers, Wines and Fruit Juices

 Lupine Publishers | Scholarly Journal of Food and Nutrition




Abstract

A post-column reaction method, which involves use of the pH indicator BTB, was developed and applied to the analysis of low molecular weight organic acids in commercial beers, wines, and fruit juices. Organic acids identified in these beverages were formic, acetic, citric, pyruvic, tartaric, malic, succinic, lactic, and pyroglutamic. Amounts of total acids in samples ranged from 980.4 mg/L to 513.5 mg/L in beers, from 7,502.3 mg/L to 5,573.3 mg/L in wines, and from 11,162.8 mg/L (orange) to 2,995.8 mg/L (mango) in fruit juices. Citric acid was found in the greatest amount-ranging from 286.7 ± 4.5 mg mg/L to 139.0 ± 5.3 mg mg/L in beers. The greatest level of malic acid was in a wine with 4,248.4 mg/L. The method developed is applicable to determine amounts of organic acids, formic and acetic acids, in beverages quickly and accurately.

Keywords: Organic acids analysis; HPLC/post-column; Beers; Fruit juices; Wines

Introduction

Low molecular weight organic acids (LMWOAs), including formic, acetic, malic, citric, tartaric, lactic, succinic, and oxalic acid, are found in various beverages, such as beers, wines and fruit juices (citrus, apple, grape, and melon), as well as in some foods [1,2]. These acids contribute characteristic tastes to beverages. For example, formic acid possesses pungent odor and sour taste in proper dilution. It is particularly adaptable to the pineapple flavor. Acetic acid also has a pungent and stinging sour odor, but it gives a clean-sour and acid taste at dilute concentrations (lower than 1%) in water and been used in flavor compositions, such as butter, chocolate, grape strawberry and wine. Malic acid is present in plum, peach, apricot and related fruits and used for imitation fruit flavors, such as maple [3]. Citric acid is the major acid component of citrus species and possesses a clean acid taste in an aqueous solution [3,4]. In addition to the role of organic acids in taste and flavor of beverages, they also play an important role in quality of beverages, including beers [5], wines [6] and fruit juices [7]. A capillary electrophoresis (CE) with spectrophotometric method achieved successfully analysis of a limited number of LMWOAs [8].

This method has been widely used for LMWOAs analysis in foods and beverages [9]. However, the resolution and detection of LMWOAs formic and acetic, achieved better by chromatographic methods than by the spectrophotometric method [10]. In the case of the most commonly used gas chromatography (GC), a tedious derivatization of organic acids is required to prepare samples for analysis because they are highly soluble in water [11]. On the other hand, HPLC, which can take aqueous samples directly, has been widely used to analyze LMWOAs in water samples; such as wine, beer [12], vinegar and fruit juices [13], coffee [14] and alcoholic beverage meads [15]. A HPLC interfaced to enzyme reactor analyzed oxalic acid in fruit and vegetable juices [16]. After the electronspray ionization system was advanced to interface between HPLC and mass spectrometers (LC/MS), LC/MS application for the analysis of water-soluble chemicals, including LMWOAs, developed significantly [17]. However, separation of LMWOAs by HPLC, formic acid and acetic acid, remains relatively difficult.

In the present study, a previously reported simple and accurate post-column reaction method for LMWOAs [18] was improved and successfully applied to analyze LMWOAs, including formic and acetic acids, in beers, wines, and fruit juices.

Materials and Methods

Beverage Samples

All commercial beverages-10 kinds of beers, 6 types of wines (5 red and 1 white), and 25 kinds of fruit juices-were bought from a local market in Seoul, Korea.

Chemicals and Reagents

Bromothymol blue (BTB, 95%), sodium phosphate dibasic anhydrous (98.5%), sodium hydroxide (98%), perchloric acid (67– 72%), oxalic acid (99.5–100.2%), phosphoric acid (85%), citric acid (99.5%), tartaric acid (99.5%), malic acid (95–100%), quinin acid (100%), succinic acid (99%), fumaric acid (99%), lactic acid (85%), formic acid (95%), acetic acid (99.7%), pyroglutamic acid (99%) were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA) or TCI Chemical Co. Ltd. (Tokyo, Japan). The purified water was prepared with a Zener Power II (Human Co., Korea). A stock solution of standard organic acids (10g/L) was prepared in purified water for preparation of standard solutions and spike analysis.

Preparation of Beer and Wine Samples

Beer and wine samples (2 mL each) were filtered with an Arcadis Syringe Filter with 0.45 mm PVDF membrane (Waters Co, Milford, MA).

Preparation of Fruit Juice Samples

Well-mixed juice samples were centrifuged at 10,000 rpm for 5min at 4 ºC to collect the supernatant. The supernatant was filtered with an Arcadis Syringe Filter with 0.45mm PVDF membrane (Waters Co, Milford, MA) and were analyzed with HPLC.

Analysis of Organic Acids in Beverages

Figure 1 shows the systematic diagram of the post-column method used in the present study. A prepared beverage sample was injected (10mL) into an Agilent 1100 model HPLC equipped with a 5cm x 8.0 mm i.d. Shorex RS pack KC-LG guard column, which was connected to two 30 cm x 8.0 mm i.d. KC-811 separation columns in series. The mobile phase was a 3mmol perchloric acid solution with a 0.7 mL/min flow rate. Temperature of the separation columns was 80ºC. The eluate from HPLC was mixed with a reaction solution containing 0.2mmol bromothymol blue (BTB), 15 mmol Na2HPO4, and 2 mmol NaOH. The reaction solution was purged at a 0.7mL/ min flow rate in a T-way connector as shown in Figure 1. Adducts formed from the acids and BTB in a 50 cm x 0.25 mm i.d. stainless reaction coil was monitored with a UV detector at l = 440 nm.

 

Results and Discussion

As mentioned above, analysis of LMWOAs is a difficult process. There have been advances in HPLC/MS methods recently but some problems, such as insufficient resolution of formic acid and acetic acid, remain. In order to resolve these problems, we developed a post-column reaction method, which involved use of pH indicator BTB. This method and related theory were originally advanced nearly three decades ago [18]. Figure 2 shows an HPLC postcolumn chromatogram of standard acids. This chromatogram shows satisfactory resolution of low molecular weight acids for quantitation. Phosphoric acid peak in this chromatogram may come from a phosphate in a reaction solution. However, phosphoric acid is not organic acid, which is out of scope of this study. The limits of detection (LOD) of acids were 25.0mg/L for citric, malic and quinin, lactic; 12.5mg/L for formic and acetic; and 50mg/L for pyroglutamic. The limits of quantitation (LOQ) of each acid were 75.0mg/L for citric, malic, quinin, and lactic; 30.0 mg/L for formic; 50.0 mg/L for acetic; and 150 mg/L for pyroglutamic.

Organic Acids Found in Beer Samples

Table 1 shows the results of LMWOAs analysis in beer samples. There are three monoprotic acids (lactic, acetic and pyroglutamic), two diprotic acids (malic and succinic), and one triprotic acid (citric acid). Amounts of total acids in beer samples varied considerably, ranging from 1,801.3 mg/L (brand E) to 776.0 mg/L (brand D). Among the acids identified in the beer samples, citric acid was generally found in the greatest amount-ranging from 286.7 ± 4.5 mg/L (brand E) to 139.0 ± 5.3 mg/L (brand G), followed by lacticranging from 274.3 ± 5.6 mg/L (brand E) to 19.3 ± 2.2 mg/L (brand G), and pyroglutamic acid-ranging from 264.9 ± 1.4 mg/L (brand E) to 105.5 ± 2.4 mg/L (brand D. ) Previously, the electrophoresis method was successfully used to analyze LMWOAs in beers. Klampft [19] found citric acid with level of 193 ± 2.4 mg/L in Chinese rice beer, 171 ± 1.5 mg/L in white beer and 178 ± 1.1 mg/L in Lager beer. Cordeiro-Ramirez et al. [20] reported citric acid (ND – 59 ± 2 mg/L), fumaric acid (ND – 5.9 ± 0.7 mg/L), malic acid (106 ± 12 mg/L), pyroglutamic acid (92 ± 6 mg/L) in 6 different beers. Citric acid exhibited synergistic effects with lactic acid-producing bacteria toward inhibition of pathogenic bacteria grow [21]. The amounts found in these acids in the present study were like these reports. For example, the present study found citric acid with amounts ranging from 146.2 ± 3.8 mg/L brand D to 286.7 ± 4.5 mg/L in brand E. In addition, the present study found pyroglutamic acid with levels ranging from 105.5 ± 2.4 mg/L in brand D to 264.9 ± 1.7 mg/L in brand E. The level of acetic acid ranged from 98.2 ± 3.0 mg/L (brand C) to not detected (brand H) in the present study, whereas acetic acid was not reported from the studies conducted with the electrophoresis method.

Organic Acids Found in Wine Samples

Table 2 shows the results of LMWOAs analysis in wine samples. The total amounts of acids were similar in all wine samples, ranging from 7,052.3mg/L (brand B) to 5,573.3mg/L (brand C). Tartaric acid was generally found in the highest concentration, ranging from 2,599.9 ± 7.5mg/L (brand F) to 1,495.1 ± 5.6mg/L (brand B). Amounts of citric acid, malic acid and lactic acid varied significantly among brands. Lactic acid was found at high levels in brands A (2,497.1 ± 2.8mg/L), C (2,271.1 ± 5.7 mg/L), and E (2,954.6 ± 4.2mg/L), but at relatively low levels in brands B (169.8 ± 1.9mg/L), D (170.4 ± 6.3 mg/L), and F (101.5 ± 4.9mg/L). On the other hand, high levels of malic acid were found in brands B (4,248.4 ± 13.8 mg/L), D (2,403.2 ± 8.0mg/L), and F (2,248.8 ± 5.3mg/L), whereas its concentrations in A was only 35.6 ± 3.8 mg/L in, C was 17.3 ± 1.4 mg/L and in E was 58.7 ± 4.2mg/L. A similar trend was observed in the case of citric acid. A previous study found lactic and tartaric acids in Ribeiro Sacra wines as the predominant organic acid components [9]. Lactic acid and tartaric acid levels ranged from 3,784mg/L to 452mg/L and 1978mg/L 866mg/L. These results are consistent to the ones from the present study. As mentioned above, the quality of wines depends on, in part, acidity associated with the composition of acids [22]. The results in the present study demonstrate that the composition of acids in different brands of wines varies. Therefore, the analytical method developed can be useful in evaluating wine quality as well as a guide to improvement in the winemaking process.

Organic Acids Found in Fruit Juice Samples

Table 3 shows the results of LMWOAs analysis in fruit juice samples. Formic acid and acetic acid, which are the lowest and 2nd lowest molecular weight acids among LMWOAs and difficult to determine by a CE method [10], were satisfactorily analyzed in the present study. The levels of formic and acetic acids found ranged from 19.8 ± 1.0mg/L (mulberry) to 4.0 ± 0.2mg/L (mandarin) and from 27.7 ± 2.7mg/L (tomato) to 17.7 ± 2.6mg/L (grape), respectively. Orange juice samples had the greatest total concentration of acids, ranging from 11,162.8 mg/L (brand D) to 8,469.5 mg/L (brand C), followed by mandarin juice samples, ranging from 10,683.1mg/L (brand C) to 5,560.6 mg/L (brand B) and grape juice samples, ranging from 5,208.2 mg/L (brand E) to 4,016.2 mg/L (brand C). Citric acid and malic acid were generally detected in higher levels than other acids, ranging from 10,270.1 ± 2.9mg/L (mandarin) to 36.9 ± 12.0mg/L (mulberry) and from 3,990.3 ± 11.6mg/L (Grape) to 66.9 ± mg/L (mango), respectively. The pear juice sample contained the lowest level of total acid with 596.2 mg/L. The total amounts of acids in mixed juices ranged from 7,237.2mg/L (brand F) to 4,105.8 mg/L (brand A), which are comparable to those of grape juices.

One previous report demonstrated satisfactory analysis of organic acids in fruit juices (apple, peach, pear and apricot) using HPLC with an ion- exclusion column [23]. Another previous study reported the amounts of citric, malic, quinin and tartaric acids in apple, orange, cranberry, white/red grapes, and pomegrante juices analyzed using LC/MS/MS [24]. Effect of malic acid for the inactivation of common food pathogens on fresh-cut lettuce was reported, suggesting that malic acid possesses some biological activity [25]. The presence of formic and acetic acids was, however, not reported in these studies. On the other hand, the present study found 9.5 ± 3.6 mg/L formic acid in pear juice, 4.0 ± 0.2 – 10.0 ± 0.8 mg/L in mandarin juice, 12.5 ± 0.5mg/L – 15.2 ± 2.4mg/L in orange juice, 13.4 ± 0.3 mg/L in grape fruit, 5.8 ± 0.5 – 10.7 ± 0.3 mg/L in grape juice, 6.6 ± 1.5mg/L in mango juice, 10.1 ± 0.7 mg/L in tomato juice, and 19.8 ± 1.0 mg/L in mulberry juice. In addition, acetic acid was determined in pear juice (8.4 ± 1.3 mg/L), grape juice (17.7 ± 2.6 mg/L – 21.1 ± 1.0 mg/L), mango (23.2 ± 0.5 mg/L), and tomato (27.7 ± 2.7 mg/L). However, it is difficult to compare the results from the present study to those from the previous study because the compositions of the juices vary in the different brands.

Conclusion

The present study demonstrates that the method developed was useful to determine LMWOAs, formic acid and acetic acid, present in samples with complex matrices of beverages. Acids composition are important to evaluate quality of beverages. Therefore, the method developed in the present study would useful to analyze LMWOAs levels for evaluating quality of beverages. The CE method is also well-established method for LMWOAs analysis. Therefore, it is recommended to use both methods, the present method for formic acid and acetic acid

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Friday, June 7, 2019

Food and nutrition journal articles - Lupine Publishers

Route Evaluation and Analytical HPLC Method Development of Buprenorphine, Naloxone, and Comparison Overall Efficiency of Naltrexone Nalbuphine Traces the Evolution of Various Approaches by Krishnasarma Pathy in Scholarly Journal of Food and Nutrition (SJFN) - Lupine Publsihers

This is out line work on the process development for the synthesis of buprenorphine, naltrexone, naloxone, and nalbuphine from naturally occurring opiates such as Thebaine, also known as codeine methyl enol ether, and oripavine. Several new methods for N-demethylation of morphinans have been developed during the pursuit of this research. The article traces the evolution of various approaches and provides a comparison for overall efficiency. A reverse phase high performance liquid chromatographic (RPHPLC) method has been developed and validated for simultaneous estimation of Naloxone Hydrochloride and Buprenorphine Hydrochloride in pure and marketed formulations. Separation was carried out using column Hypersil ODS C18 (250 mm x 4.6mm x 5μm particle size) in isocratic mode using mobile phase composition pH 6.0 ammonium acetate Buffer: Acetonitrile (68:32)v/v and UV detection at 310nm. The compounds were eluted at a flow rate of 1.0mL/ min. The average retention times for Naloxone and Buprenorphine were 2.86 and 3.67 min, respectively.

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Wednesday, April 10, 2019

Journal of Food and Nutrition - Lupine Publishers

The Loop Regions and Substrate Specificity of GH 27 Familiy α-Galactosidases by Canfang Niu and Peilong Yang’s in Scholarly Journal of Food and Nutrition (SJFN) -  Lupine Publishers

Galacto-oligosaccharides (i.e. stachyose and raffinose) commonly exist in food and feed and are indigestible by human and animals, thereby causing flatulence, gastrointestinal disturbance and low feed efficiency. [1] α-Galactosidases (EC. 3.2.1.22) has hydrolysis ability to degrade these anti-nutritional factors, decrease the viscosity of the diet, reduce the occurrence of diarrhoea, destroy the structure of the cell wall of the plant, promote the nutrition release, improve the utilization efficiency of the nutrients in the feed, increases lean meat rate, enhances immune function and disease resistance of animals. [2,3] α-Galactosidases has great application value in industrial processes of feed, food, and beet sugar production. α-Galactosidases are widely distributed in fungi, bacteria, plants and human (www.cazy.org). Fungal α-galactosidases have maximal activity at pH 3-5, but bacterial α-galactosidases are the optimal pH of 6-7.5.1.

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Blissful Thanksgiving!!!

Greetings from Scholarly Journal of Food and Nutrition (SJFN) Wishing you a harvest of blessings, good health and good times. Happy Thanksgi...